What is HybISS?
HybISS (Hybridization In Situ Sequencing) is a Padlock-probe based assay in which probes hybridise onto mRNA, close by ligation, are amplified by rolling circle and read out by hybridisation with fluorescent probes. It is a powerful technique, but a single design error in one probe can ruin the whole batch. This tool audits every design before you buy or synthesise anything.
How it works
1. Upload
Upload probe batches in PDF, Excel, CSV or FASTA. Processing happens locally in your browser.
2. Quality control
The app applies automatic rules: arm lengths, nick gaps, mismatches and IUPAC nomenclature.
3. Topological rendering
Interactive visualisation of each probe's structure — coming soon.
4. Export
Download reports in CSV, Excel or vector graphics ready for publications and reviews.
Who it is for
Researcher / Bioinformatician
Uploads bulk batches, audits QC reports and exports vector graphics for publications.
Laboratory technician
Uses the platform as a checklist to cross-check supplier synthesis sheets against the original design before reconstituting the probes.
Student / Teacher
Uses the interactive view to understand the hybridisation mechanism and assembly of the HybISS complex.